89 3 Responses of alveolar-like macrophages to lysine deacetylase inhibition Supplementary Figure 2: Effect of KDAC inhibitor pre-treatment on mitochondrial respiration in alveolarlike macrophages. Bars quantify mitochondrial respiration of macrophages when treated with KDAC inhibitors. Data represent seven biological replicates (n=6 separate wells per condition). Mitochondrial parameters were compared among treatments and significance was calculated by Friedman test with a Dunn’s correction for multiple testing for CTRL vs KDAC inhibition. All error bars are shown as mean ± SD. Supplementary Figure 3: Protein expression of metabolic enzymes in alveolar-like macrophages after KDAC inhibitor treatment in combination or not with LPS. Cells were incubated with KDAC inhibitors (MS275/ RGFP966) with or without LPS (LPS, control) for 16 h. After 16 h cells were stimulated with LPS (10 ng/mL). Protein expression levels were measured with a targeted approach, using concatenated peptides as internal standards for protein quantification. Data are represented as radar plots (as percentage of control, n=6). (A) Proteins belonging to the glycolytic pathway, (B) proteins belonging to the connection between glycolysis and the TCA cycle as part of the pyruvate dehydrogenase complex, (C) proteins belonging to the TCA cycle, (D) proteins belonging to the fatty acid oxidation processes (FAO), and (E) proteins belonging to the electron transport chain (see Figure 5 for details).
RkJQdWJsaXNoZXIy MTk4NDMw