4 DNA damage response regulates RAG1/2 expression through ATM-FOXO1 | 111 Supplemental Figure 2. Single antibody specificity controls for Proximity Ligation Assay. BV173 cells treated overnight with 5 mM STI571 and subsequently treated with 50 ng/mL neocarzinostatin for 2 hrs. Cells were cytocentrifuged and incubated overnight with the indicated single antibodies and developed as indicated in Figure 5. In none of the single antibody controls >5 specific PLA signals were observed in the DAPI-stained nuclei. Supplemental Figure 3. Expression levels of MAPKAPK5 and BCL11a are not affected by DNA damage in STI571-treated BV173 pre-B cells. BV173 cells treated overnight with 5 mM STI571 and subsequently treated with 50 ng/mL neocarzinostatin for 2 h. Western blot analysis for MAPKAPK5 and BCL11a protein levels. The β-actin blot was used to control for loading.
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