5 NF-κB and AKT signaling prevent DNA damage by suppressing RAG1/2 | 141 Supplemental Figure 4. Expression of IkBa super-repressor in mouse Abl pre-B cells inhibits LPS-induced upregulation of CD69 expression. CD69 expression was determined in LPS-treated (25 mg/mL; 24h) mouse Abl pre-B cells retrovirally transduced with IkBaSR-IRES-YFP. (A) Overlay FACS histograms are shown of untreated cells and LPS-stimulated cells gated on YFP-positive cells and YFP-negative cells. (B) Graph depicts CD69 mean fluorescence intensity within IkBaSR negative (YFP-negative) and IkBαSRpositive (YFP-positive) gates. Each symbol represents an individual stimulation (n=4)
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