6 DNA damage-induced p53 downregulates expression of RAG1 | 161 perform the stainings: rat-anti-mouse CD45R/B220 (BD Pharmingen, San Diego, CA, clone RA3-682) rat-anti-mouse CD43(BD Pharmingen, clone S7), rat-anti-mouse Ig kappa light chain (BD Pharmingen, clone 187.1), rat-anti-mouse IgM (eBioscience, San Diego, CA, clone II/41), rat-anti-mouse IgD (Southern Biotech, Birmingham, AL, clone 11-26), rat-anti-mouse CD49b (BD Pharmingen, clone DX5), rat-anti-mouse Ly-6C (BD Pharmingen, clone AL-21). Retroviral transductions LZRS-FOXP1-IRES-YFP (FOXP1 FL) and LZRS-BCL6-IRES-GFP (BCL6) were generated as previously described9,10. A FOXP1-iso construct that starts translation from the second coding ATG (in exon 6; M101), encoding a 100 AA N-terminally deleted FOXP1, was PCR-cloned and subcloned into LZRS-IRES-YFP (FOXP1 iso). Cells transduced with an empty LZRS-IRES-YFP were used as a control. The pCL-ECO-miR-34a-sponge and pCL-ECO-scrambled were kindly provided by Dr. J. Kluiver, University Medical Center Groningen, The Netherlands. The viral transductions were performed as previously described9–11. Transduced cells were FACS-sorted based on GFP expression 5 d after transduction.
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