164 | Chapter 6 Supplemental Figure 3. (A) Rag1 and Rag2 mRNA expression measured by RT-PCR in mouse v-Abl WT cells (A70) transduced with either empty vector (LZRS), human full-length FOXP1 (FOXP1 FL), the isoform of human of FOXP1 (FOXP1 iso) or human BCL6. The transduced cells were not treated with STI571, therefore this expression reflects the Rag1 and Rag2 mRNA expression before the alleviation of the developmental block at the pre-to-pre-B cell stage. (n=2, mean±SD, *p<0.05). (B) Rag2-/- mouse v-Abl cells were transduced with either empty vector (LZRS), human full-length FOXP1 (FOXP1 FL), the isoform of human of FOXP1 (FOXP1 iso) or human BCL6, the formation of coding joints was assessed by qualitative PCR of Vκ6-23 to Jκ1 joint, using 100ng and 500ng DNA, showing that the absence of a PCR product is not a result of too little DNA input in the PCR reaction. Control mouse WT v-Abl cells (A70) were treated with STI751 to induce RAG1/2 expression and recombination at Igk. The PCR products were run in 2% agarose gel containing ethidium bromide.
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