3 RAG1/2 introduces double-stranded breaks at non-Ig loci | 63 genes previously associated with B-cell malignancies in mouse and human, such as Ikzf4, Abl2, Klf4, Tcf4, Tcf7, Rassf8, Mef2c33–39 or involved in DNA repair, Rad51ap140 (SUPPL Table 2). Figure 3. (A) Pie chart showing the distribution of RAG1/2-dependent NBS1 peaks according to position within genes. (B) Pie chart showing the distribution of RAG1/2-dependent NBS1 peaks according to gene categories. Of the 1489 RAG-dependent NBS1 peaks, 1473 peaks were annotated, of which 45,9% (674 of 1473) were associated with known genes. Of all the peaks, 4% (59 of 1473) were located in the gene exons and 30% (443 of 1473) were located in the gene introns (Figure 3A). 92.7% of genes that contained NBS1 peaks were protein-coding genes, the rest of the genes (7.3%) containing NBS1 peaks were regions coding for miRNA, rRNA, lincRNA, or other types of RNA (Figure 3B). Finally, 799 of 1473 RAG-dependent NBS1 peaks were not associated with any known genes.
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