ERRATUM II Chapter 4, page 93, Figure 2 should be displayed as follows: Figure 2. RAG1 down-regulation upon DNA damage is caspase-3-independent, requires its endogenous transcriptional context, and coincides with loss of FOXO1-Erag binding. (A) Western blotting analysis of RAG1 and cleaved caspase-3 expression in STI571-treated BV173 BCR-ABLpositive B-ALL cells. Cells were pre-treated with 10 M Q-VD-OPH 1h prior to 2h treatment with 50 ng/mL NCS treatment. The -actin blot was used to control for loading. (B) Western blotting analysis of HEK293 cells transfected with pEBB-RAG1 encoding full-length mouse RAG1 (kind gift from Dr. M. van der Burg). Cells were treated as indicated and harvested 2h after induction of DNA damage. The phospho-p53 (Ser15) blot was used to show the induction of the DNA damage response. The -actin blot was used to control for loading. (C) Schematic depiction of the human RAG1/2 locus, numbers indicate chromosomal coordinates and arrows indicate transcriptional direction. Erag enhancer and ChIP-PCR target region are shown. (D) Binding of FOXO1 and control rabbit IgG, and (E) phospho-ATM (Ser1981) and control mouse IgG to the Erag enhancer in STI571-treated BV173 cells and SUP-B15 BCR-ABL-positive B-ALL cells as determined by ChIP and expressed as percent of input DNA. Cells were treated as indicated. ChIP experiments were performed in triplicate. Statistical significances were determined by one-way analysis of variance (ANOVA) using a Bonferroni’s post-test, means ± SEM are shown (** p<0.01; *** p<0.001).
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