2 Cystinosis (ctns) zebrafish mutant shows pronephric glomerular and tubular dysfunction 25 Figure 3. Early developmental stages of zebrafish ctns‒/‒ embryos and response to cysteamine therapy. Embryonic development was monitored over the first 3 days of life at predetermined time points: (a) 3hpf, (b) 6hpf, (c) 24hpf, (d) 48hpf and (e) 72hpf. The outcomes of four different mating settings, 16 females and eight males from each genotype were used (363 ctns‒/‒ and 322 wt embryos). Percentages of different developmental stages at each time point were calculated per the total number of living embryos for each genotype at each time point. * P<0.05, *** P<0.001 against wildtype percentages using Pearson chi-square test. (f) Effect of different doses of cysteamine therapy on mortality rates of ctns‒/‒ larvae during the first 96 hpf. * P<0.05, ** P<0.01, *** P<0.001 against untreated ctns‒/‒ larvae using student's t test.
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