Chapter 2 28 Figure 5. Morphology of the pronephros of ctns‒/‒ larvae compared to the wt. (a) H&E stained cut-section of a 6 dpf wt larva at the level of the glomerulus and proximal tubules(bar=50µm). (b) H&E stained cut-section of a 6 dpf ctns‒/‒ larva at the level of the glomerulus and proximal tubules showing no apparent abnormality (bar=50 µm). (c) Block face scanning EM image of the proximal tubule of a 4 dpf wt larva (bar=5 µm).Demarcated area was magnified (right) to show size and distribution of lysosomes (asterisks) in the wt (bar=2 µm).(d) Scanning EM image of the proximal tubule of a 4 dpf ctns‒/‒ larva showing intact brush border (bar=5 µm). Demarcated area was magnified (right) to show larger number of lysosomes (asterisks) many of which were significantly enlarged in size compared to the wt (bar=2 µm). (e) Quantitation of the number and surface area of lysosomes in cut sections at the level of proximal tubules in
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