3 Glomerular permeability is not affected by heparan sulfate glycosaminoglycan deficiency in zebrafish embryos 53 negatively charged sites and form electron-dense deposits. Consistent with our hypothesis, the mean number of electron-dense deposits per μm of GBM was significantly lower in homozygous dak/ext2 mutants compared to WT embryos (0.80, SD 0.39, n = 2 versus 2.2, SD 0.72, n =2, respectively; p<0.05) (Figure 2). Figure 1. Homozygous dak/ext2 mutant zebrafish develop morphological changes Shown are examples of a wild-type (A) and homozygous dak/ext2 (B) embryo at 5 dpf, with several morphological changes in the mutant embryos. Arrows 1, 2, and 3 indicate a protruding jaw, pericardial edema, and a non-inflated swim bladder, respectively; arrow 4 shows a normally developed swim bladder in the WT embryo. Scale bar = 250 µm. Foot process width Ultrastructural analysis of the GFB using transmission electron microscopy revealed normal foot processes (Figure 2) in homozygous dak/ext2 mutants. The mean width of foot process did not differ significantly between homozygous dak/ext2 mutants and WT embryos (0.181. SD 0.025, n = 3 versus 0.176, SD 0.012, n = 3; p=0.76).
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