Chapter 3 54 Figure 2. Homozygous dak/ext2 mutant zebrafish have significantly fewer electron dense-deposits in the GBM, but normal podocyte foot process width Transmission electron micrographs of the glomerulus (A and B), a capillary loop (C and D) and representative sections of GBM (E and F) in a wild-type (A, C, and E) and homozygous dak/ext2 mutant (B, D, and F) embryo. Sections were stained with PEI particles, which bind to negatively charged molecules in the GBM, forming electron-dense deposits (examples indicated by white circles in E and F). (G) Summary of the number of electron-dense PEI-containing deposits in the GBM; *p<0.05. (H) Summary of the foot process width; ns, p>0.05. Scale bars = 10 µm (A and B), 1 µm (C and D) and 200 nm (E and F). Glomerular permeability Next, we analyzed the reabsorption of injected dextran particles (Figure 3). We found no significant difference between homozygous dak/ext2 mutants and WT embryos with respect to the mean number of reabsorbed 3-kDa dextran droplets (22.69, SD 10.22, n = 32 versus 24.33 SD 12.51, n = 21; p=0.60). PAN-injected WT embryos, which were used as a positive control, had a similar number of 3-kDa dextran droplets (25.79, SD 13.33,
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