Chapter 5 92 Ctsl mRNA levels—but not dynamin protein area expression—are higher in Dahl rats compared to SHR rats Cathepsin L, encoded by the Ctsl gene, is an enzyme that cleaves dynamin.(13) We therefore measured the expression of cathepsin L in the glomeruli of Dahl and SHR rats using RT-PCR (Figure 1I). We found that Ctsl mRNA levels were significantly higher in the glomeruli of Dahl rats compared to SHR, ranging from 1.3-fold to 1.7-fold higher, at all ages tested (p < 0.01). Immunohistochemistry revealed that there was no difference between Dahl and SHR rats in glomerular cathepsin L positive area. (p = 0.11 and p = 0.70 at 4 and 6 weeks of age respectively, Figure 1H) Figure 1. Glomerular Dnm2 and Dnm1 mRNA levels—but not dynamin protein levels—are increased in Dahl rats prior to the onset of proteinuria
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