109 Simultaneous fluorescence imaging of bowel perfusion and ureter delineation using methylene blue Table 3. Mean TBR values for different time points among different MB dose groups TBR ± SD Group 1 (0.75mg/kg) Group 2 (0.50mg/kg) Group 3 (0.25mg/kg) p value* TBR 5 1.95 ± 1.46 0.92 ± 0.06 1.21 ± 0.04 0.826 TBR 10 3.45 ± 3.46 4.31 ± 1.84 5.16 ± 0.77 0.617 TBR 20 4.20 ± 2.52 6.45 ± 2.02 4.46 ± 0.71 0.374 TBR 30 4.76 ± 2.92 6.18 ± 1.17 5.04 ± 1.36 0.178 TBR 40 6.39 ± N/A 6.14 ± 2.55 4.48 ± 1.37 0.544 TBR 50 4.66 ± 0.42 6.40 ± 0.19 4.11 ± 0.26 0.405 TBR 60 6.76 ± 0.49 6.27 ± 0.22 4.37 ± 0.13 0.356 TBR 80 4.77 ± 0.14 6.04 ± 4.02 5.46 ± 1.87 0.760 TBR 100 5.00 ± 3.38 5.11 ± 0.67 4.09 ± 0.17 0.812 TBR 120 5.00 ± 1.28 4.29 ± 0.60 5.60 ± 2.02 0.751 Mean (95% CI) 4.46 ± 0.376 (3.31 – 5.62) 5.21 ± 0.37 (4.01 – 6.40) 4.38 ± 0.37 (3.18 – 5.57) 0.345 TBR, Target to Background ratio; *Univariate tests within mixed model analysis Bowel perfusion analysis In all pigs, a clear macroscopic NIRF visualization of the perfusion in ICG and MB mode was achieved in all pigs within a few seconds after dye administration (Figure 3). After 20 seconds, maximal intensities were reached (Figure 4). No interference between ICG and MB was observed when switching to either mode. In pigs 4 and 5, bowel perfusion was assessed every 10 minutes for at least 60 minutes to investigate washout and fluorescence intensities over time. After 50 minutes of dye administration, ICG was still clearly visible while MB was almost no longer visible in pig 4 (MB dose of 0.5 mg/kg) (Figure 5). After 60 minutes, only a few spots of MB dye were still visible. Pig 5 (MB dose of 0.25 mg/kg) also showed an earlier washout time of MB compared to ICG, after 40 minutes of MB injection. No adverse reactions were observed in any of the animals after ICG administration. 5
RkJQdWJsaXNoZXIy MTk4NDMw