128 Chapter 6 Secondly, the fluorescence quantification analysis of all images of the ischemic loop during ICG administration was performed (T = 60 min). All ROIs showed a similar development of increase in fluorescence intensity as during MB analysis (Figure 3D). Compared to MB, absolute values were higher. Local lactate levels of ROI 3 also had lower levels compared to all other areas (Figure 3E) and most of the values were higher than at T = 10 min. A second Spearman’s correlation test showed a significant negative correlation between ingress levels in the ischemic bowel loop and corresponding local lactate levels (r = -0.5367, 95% CI: -0.7965 to -0.1096; p = 0.015) for ICG fluorescence quantification analysis (Figure 3F). Figure 3. Results of bowel perfusion analysis. A-B-D-E values present medians, whiskers indicate the 75th percentile. (A) Ingress values of the 5 ROIs during MB administration (T = 10 min). (B) Local lactate levels of the 5 ROIs during MB administration (T = 10 min). (C) Scatterplot of ingress values and local lactate during MB administration showing a significant negative correlation (Spearman’s rho = -0.7709, 95% CI: -0.9073 to -0.4878; p = <0.001). (D) Ingress values for the 5 ROIs during ICG administration (T = 60 min). (E) Local lactate levels of the 5 ROIs during ICG administration (T = 60 min). (F) Scatterplot of ingress values and local lactate during ICG administration showing a significant negative correlation (Spearman’s rho = -0.5367, 95% CI: -0.7965 to -0.1096; p = 0.015). All detailed information is provided in Supplementary S2. In one animal, NIRF imaging was performed of a non-ischemic bowel loop after injecting MB and ICG to compare absolute ingress values with the same method of administration, at the same timing. The ingress values were 7.02 i/s and 11.89 i/s for MB and ICG, respectively. No adverse reactions were observed in any of the animals after MB and ICG administration.
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