143 Real-time intestinal perfusion assessment for anastomotic site selection using LSCI enabled all five surgeons to accurately identify the differences, resulting in a 100% accuracy rate. When asked to identify the best perfused loop, four out of five (80%) surgeons provided correct answers based solely on the laser speckle images. Figure 2. (A) The white light image of the three differently perfused loops. * Indicates the ischemic loop, ** indicates the compromised loop and *** indicates the healthy loop. (B) The PerfusiX-Imaging® perfusion image with the three differently perfused loops. Blue indicates low perfusion and yellow indicates high perfusion. * Indicates the ischemic loop, ** indicates the compromised loop and *** indicates the healthy loop. (C) The white light image of the anastomosis with a bad perfused segment indicated by + and an uncompromised segment indicated with ++. (D) The PerfusiX-Imaging perfusion image of the anastomosis. Blue indicates low perfusion and yellow indicates high perfusion. The compromised segment indicated by + and an uncompromised segment indicated with ++. Identification of anastomotic perfusion The study’s findings demonstrated that the LSCI perfusion images had an impact on the surgeons’ decision-making concerning anastomosis creation. All five senior surgeons, when presented with LSCI feedback (Figure 2B and D), recommended against creating an anastomosis, resulting in a recommendation rate of 100%. In terms of identifying perfusion differences, the LSCI feedback alone proved to be highly effective, as all surgeons correctly identified the differences, leading to a 100% accuracy rate. Similarly, when asked to identify the worst perfused tissue, all surgeons provided correct answers based solely on the LSCI feedback. The inclusion of white light images did not alter the accuracy in this regard. 7
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