161 Real-time quantification of LSCI during intestinal laparoscopic surgery Figure 3. Overview of both spatial (A-D) and temporal (E) progression of average laser speckle perfusion units (LSPUs) per Region of Interest (ROI). A) LSPUs per ROI before devascularization (T-1). There was no significant difference between the ROIs. B) LSPUs measured at the ROIs directly after devascularization (T0). C) LSPU measurements after 60 minutes of ischemia (T60). Values further diverge and become more significant. D) At 120 minutes after inducing ischemia to the intestinal segment (T120), differences between all ROIs are highly significant. Dashed and dotted lines indicate mean values and quartiles respectively. E) Temporal development of LSPUs per ROI. A strong decline can be seen in ischemic tissue, shortly after inducing ischemia, further decreasing after this. As time progresses, the ischemic ROIs show increasingly low values compared to other ROIs. In addition, 95% CI bars narrow when ischemic time increases. Levels of significance in all images (P-values): *: ≤0.05; **: ≤0.01; ***: ≤0.001. An overview of all P-values can be found in Supplemental 2. 8
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