167 Real-time quantification of LSCI during intestinal laparoscopic surgery CONCLUSION This study demonstrates the accuracy of Laser Speckle Contrast Imaging in visualizing and distinguishing between ischemic and well-perfused tissue. Changes in Laser Speckle Perfusion Units corresponded with alterations in lactate levels in both types of tissue. A cut-off value of 69 for LSPU showed high sensitivity and specificity. LSCI holds promise as an adequate and objective perfusion visualization tool, but further research on real-time quantification of LSPUs and clinical applicability is imperative. ACKNOWLEDGEMENTS AND STATEMENTS The authors would like to express their gratitude to the staff of the animal laboratory of the Maastricht University Medical Centre (MUMC+, Maastricht, The Netherlands) for their help during the animal study and the staff of the Department of Epidemiology of the Medical Centre Leeuwarden for their help during the data analysis. This research received no external funding. PerfusiX-Imaging® equipment was provided by LIMIS Development BV, Leeuwarden, The Netherlands. 8
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