260 Chapter 11 Genetic Analysis Methods Primary tumor genetic analysis was performed on DNA level in 19 studies and on RNA level in seven studies (Figure 5). One study described the analysis on both levels 35. Heublein et al. investigated MicroRNAs (miRNA) and the corresponding overexpression profiles 24. Four articles did not specify if they performed testing on DNA or RNA level 43, 46, 47, 52; three of these articles did not even specify which method they used for genetic testing 43, 46, 52. Nine articles reported the use of real-time polymerase chain reaction (RT-PCR) 20, 21, 24, 31, 32, 35, 38, 42, 48. One study specified the PCR tool as quantitative methylation-specific PCR (qMSP) 41. PS and NGS were used in one 37 and five 23, 28, 30, 34, 50 studies, respectively. Christensen et al. reported the use of both methods 44. Two studies analyzed the samples with SS 47, 49. Jacob et al. described NanoString analysis in both their articles 25, 26. All details about the genetic analyses are displayed in Table 3. A B Figure 5. Distribution of (A) genetic analysis level and (B) different molecular techniques.
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