3. Biodistribution of needle-injections and needle-free jet-injections visualized by a 3D- Fluorescent Imaging Cryomicrotome System 80 RESULTS A total of 30 samples (21 keloid, 9 normal skin) were analysed for the biodistribution of TCA injected with a hypodermic needle and jet injector. The keloids were obtained from six patients who underwent elective keloid excision and adjuvant radiotherapy, and were located on the abdomen, chest, mandibula or shoulder. Normal skin was obtained from three patients who underwent abdominoplasty. Fluorescent TCA volume A large variation in fluorescent TCA volumes was observed in keloids (Figure 3a, 3b). With the perforation technique, the mean fluorescent TCA volumes were similar (975 l ± 284) compared to the ‘conventional’ needle injections (990 l ± 479). Considerable operator injection force was needed for the needle injections and changing the needle position was sometimes necessary to inject the predefined volume of 100 µl. With the jet injector, only a single attempt was made to inject the TCA. With the jet injector, the fluorescent TCA volume in keloids (401 l ± 219) and normal skin (249 l ± 59) seems to be smaller compared to needle injections (Table 1, 2). A. B. Figure 3. Fluorescent TCA volumes A. Fluorescent TCA volumes in keloid and normal skin after needle injections. B. Fluorescent TCA volumes in keloid and normal skin after jet injections.
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