3. Biodistribution of needle-injections and needle-free jet-injections visualized by a 3D- Fluorescent Imaging Cryomicrotome System 88 Level of needle injection Mean TCA volume (L) Normal skin (n = 3) Middermal1 872 ± 227 Keloid (n = 3) Superficial1 1069 ± 516 Mid1 745 ± 159 Deep1 1158 ± 697 Perforation2 975 ± 284 Table 1. Mean fluorescent TCA volumes in mid-dermis of normal skin; and superficial, mid, and deep layers of keloid after needle injection. 1 Standard technique, i.e. using one injection for TCA administration. 2 ’Perforation technique’, i.e. making multiple cross-sectional passes with a thick needle prior to injection in the mid-layer of the keloid. Specified data is reported in Supplement 1. Jet pressure (Bar) Mean TCA volume (l) Mean residual volume (%) Normal skin (n = 3) 4 210 ± 59 28 ± 24 6 287 ± 59 27 ± 8 Keloid (n = 3) 4 329 ± 447 59 ± 12 5 384 ± 184 80 ± 12 6 489 ± 27 89 ± 20 Table 2. Mean fluorescent TCA volumes and residual volumes in normal skin and keloid samples after jet injection. Specified data is reported in Supplement 2.
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