Karlijn Hummelink

Chapter 3 120 Table S1. Continued Sample ID 1 = DC 6m 0= PD 1 = DC 12m, 0= PD T= Training, V= Validation CD8 TILs per mm2 IT-CD8 T cells (%) PD-1T TILs per mm2 CD3+ positive area per mm2 TLS per mm2 TLS+LA per mm2 CD20+ positive area per mm2 NKI-131 0 0 V 153 42.38 29 0.87 0.00 0 0.08 NKI-132 1 0 V 274 4.73 NA 2.21 0.00 0.00 1.30 NKI-133 0 0 V NA NA NA NA NA NA NA NKI-134 1 0 V 927 54.17 NA 4.66 0.00 0 0.07 NKI-135 0 0 V NA NA NA NA NA NA NA NA, not available Table S2. Predictive accuracy of individual and composite biomarkers at a prespecified criterium of ≥90% sensitivity for detecting disease control (DC) at 6 months in the training cohort. * Indicates an interaction term. Model Sensitivity ≥90% Specificity Specificity 95% CI lower limit Specificity 95% CI upper limit PD-1T * LA 0.92 73 54 88 PD-1T * PD-L1 0.92 73 54 88 PD-1T * CD3 0.92 73 54 88 PD-1T * IT-CD8 0.92 73 54 88 PD-1T * CD20 0.92 70 51 85 PD-1T 0.92 70 51 85 PD-1T * TLS 0.92 70 51 85 CD8 * IT-CD8 0.94 62 45 77 PD-1T * TIS 1.00 60 36 81 LA * TIS 1.00 55 32 77 TIS 1.00 55 32 77 CD3 * TIS 1.00 55 32 77 CD3 * IT-CD8 0.94 54 37 71 PD-1T * CD8 0.92 53 34 72 TLS * CD20 0.94 51 34 68 LA * CD20 0.94 51 34 68 TLS * TIS 1.00 50 27 73 CD20 * TIS 1.00 50 27 73 CD20 0.94 49 32 66 IT-CD8 * TIS 1.00 45 23 68

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