Chapter 6 218 diagnostic assays. EGFR T790M was confirmed in all four matched supernatants and cell pellets. Two out of the seven samples were initially taken specifically for molecular resistance analysis by NGS/TSACP V1.0 (MiSeq; Illumina, San Diego, CA, USA) using the cell pellet: in one of these samples NGS could be successfully performed but no T790M mutation was found, while for the other sample in the end no NGS was done due to a low estimated tumor cell percentage. Both samples had tumor cell percentages ≥5% (Table S1). Table 2. Detection of original driver mutations by droplet digital PCR in paired samples (supernatant and cell pellet available) of pleural effusions. Supernatant Cell pellet Driver positive Driver negative Total Driver positive 30 5 35 Driver negative 1 8 9 Total 31 13 44 Data are presented as n. Table 3. Detection of EGFR T790M by droplet digital PCR in paired samples (supernatant and cell pellet available) of pleural effusions Supernatant Cell pellet T790M positive T790M negative Total T790M positive 7 0 7 T790M negative 0 13 13 Total 7 13 20 Data are presented as n.
RkJQdWJsaXNoZXIy MTk4NDMw