162 Chapter 7 Supplementary Table 2. Antibiotic-induced gut microbiome disruption does not alter mediator release following HDM/LPS challenge in patients with asthma and HDM allergy control antibiotic treatment saline n = 13 HDM/LPS n = 13 saline n = 7 HDM/LPS n = 7 IL1-RA 844.33 [647.81, 1992.32] 7878.75 [3164.76, 12732.69]* 1005.13 [412.18, 1660.22] 6739.11 [5433.27, 12732.69]* IL-1ß 3.35 [1.43, 6.59] 25.65 [9.02, 34.36]* 0.82 [0.61, 3.90] 32.51 [19.35, 45.56]* IL-2 2.12 [2.12, 12.16] 42.67 [5.79, 62.74]* 2.12 [1.50, 2.36] 76.34 [48.50, 96.83]* IL-6 11.90 [4.12, 88.51] 685.96 [151.92, 1226.14]* 5.39 [2.76, 11.64] 903.36 [734.88, 1158.78]* IL-8 88.48 [38.61, 300.98] 729.67 [255.93, 1323.62]* 56.85 [28.70, 152.32] 1462.61 [805.19, 1462.61]* IL-33 4.75 [3.44, 6.89] 11.91 [8.16, 14.18]* 1.89 [1.20, 10.09] 13.56 [12.12, 18.95] MPO 9003.70 [5992.75, 15566.26] 25627.16 [19921.36, 30801.68]* 5782.06 [3617.93, 12956.17] 26806.04 [23866.56, 35545.16]* Periostin 100.63 [100.63, 693.54] 903.74 [693.54, 1640.81]* 100.63 [100.63, 797.42] 693.54 [325.28, 1866.45] RANTES 5.09 [3.09, 8.44] 17.60 [6.75, 22.98]* 6.18 [3.13, 9.23] 15.34 [13.19, 16.22] TNF-α 0.37 [0.18, 4.26] 37.49 [7.90, 105.92]* 0.25 [0.18, 1.07] 136.31 [91.12, 188.33]* Data are presented as median and interquartile ranges (IQR, Q1-Q3). Bronchoalveolar lavage fluid concentrations of cytokines and chemokines. Analytes were chosen based on previous experiments which a similar study design, where among others Interleukin (IL)-4, IL-5 and IL-13 were not reliably measurable with many samples below the lower limit of detection1. Eotaxin-1 and Granulocyte-macrophage colony-stimulating factor (GM-CSF) were below the limit of detection and excluded from analysis. House dust mite (HDM); Interleukin (IL)1-Receptor Antagonist (IL1-RA); Lipopolysaccharide (LPS); Myeloperoxidase (MPO); Regulated on activation, normal T-cell expressed and secreted (RANTES); Tumor Necrosis Factor (TNF)-α. Data were analyzed using a paired Wilcoxon signed rank sum test. *Denotes statistical significance (P<.05) versus saline. Differences between antibiotic treated and control patients were not significant (data not shown).
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