262 Chapter 11 Table 1. Methods applied on positive BCs [8, 13-20, 28-30] Kit/system (manufacturer) Principlea TATb (h) Panelc Microflex LT (Bruker Daltonics) Vitek MS (bioMérieux) MALDI-TOF 0.2-0.5 Not limited PNA-FISH/ QuickFISH (AdvanDx) Fluorescent in situ hybridization with PNA probes 1.5-3/ 0.5 4 different panels (S. aureus/CoNS; E. faecalis/Enterococcus spp.; E. coli/K. pneumoniae/P. aeruginosa; Candida spp.) Xpert MRSA/SA Blood Culture (Cepheid) Multiplex real-time PCR 1 S. aureus/CoNS GeneOhm StaphSR (BD) Multiplex real-time PCR 1-1.5 S. aureus/CoNS Prove-it Sepsis (Mobidiag) Broad-range PCR + microarray-based detection 3 60 bacteria, 13 fungi FilmArray Blood Culture Identification [BCID] (bioMérieux) Multiplex real-time PCR 1 8 GP, 11 GN, 5 Candida Verigene GramPositive Blood Culture [BC-GP] (Luminex) Multiplex PCR + solidmicroarray detection 2.5 11 GP Verigene GramNegative Blood Culture [BC-GN] (Luminex) Multiplex PCR + solidmicroarray detection 2 9 GN Sepsis Flow Chip (Master Diagnostica) Microarray-based assay 3 7 GP, 10 GN, 1 Candida Accelerate Pheno System (Accelerate Diagnostics) FISH (ID) Morphokinetic cellular analysis (AST) 1.5 (ID) 7 (AST) 6 GP, 8 GN, 2 Candida a ID, identification; AST, antimicrobial susceptibility testing. b TAT, turn-around time (in hours). c CoNS, coagulase-negative staphylococci; GN, Gram-negative bacteria; GP, Gram-positive bacteria. d AMK, amikacin; AMP, ampicillin; ATM, aztreonam; CAZ, ceftazidime, CFT, ceftaroline; CIP, ciprofloxacin; CRO, ceftriaxone; DAP, daptomycin; ERT, ertapenem; ERY, erythromycin; FEP,
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