264 Chapter 11 Table 2. Commercially-available tests for pathogen detection directly from blood [7, 9, 13-15, 23-25] System (manufacturer) Principle TATa (h) Blood (ml) Panelb Resistance genes LoDc (CFU/ ml) Sensitivity (%) Specificity (%) Cost Instrument Test SepsiTest (Molzym) Broadrange PCR + sequencing 8-12 2 × 1 >345 bacteria and fungi - 20-460 21-87 85-96 €€ €€€ SeptiFast (Roche Molecular Diagnostics) Multiplex real-time PCR 4-6 1.5 9 GP, 10 GN, 6 fungi mecA 3-30 25-86 74-97 €€ €€€ MagicPlex Seegene) Multiplex real-time PCR 3-5 1 73 GP, 12 GN, 6 fungi mecA, vanA, vanB 30 37-65 66-92 €€ €€€ VYOO (Analytic Jena) Multiplex PCR + gel electrophoresis 7-8 5 14 GP, 18 GN, 7 fungi mecA, vanA, vanB, blaSHV 3-10 60 70-75 €€ €€€ Iridica (Abbott Molecular) Multiplex PCR + ESI-MS 6-8 5 >750 bacteria and fungi mecA, vanA, vanB, blaKPC 4-16 50-91 79 €€€€ €€€ T2Candida panel (T2 Biosystems) PCR + magnetic resonance technology 3-4 4 5 Candida - 1 91-100 98 €€€ €€€ a TAT, turnaround time (in hours). b GN, Gram-negative bacteria; GP, Gram-positive bacteria. c LoD, limit of detection (in CFU/ml).
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