116 Chapter 4 species of “Ca. Methylomirabilis lanthanidiphila” (100% AAI to the type genome (Versantvoort et al., 2018), and the latter was a novel species sharing only 81% AAI to “Ca. Methylomirabilis limnetica” (Supplementary Figure 1 and 2). The genome-to-genome distance between MAG 38 and “Ca. M. limnetica” ranged from 0.15-0.59, with a probability of DNA-DNA hybridization > 70% ranging from 0-0.08%, supporting AAI results. We have named this novel species “Ca. Methylomirabilis tolerans” due to its persistence over the sulfide and NO stress experiment (Figure 3). Genome analyses indicated that “Ca. Methylomirabilis tolerans” had a similar metabolic potential for NO dismutation coupled to methane oxidation via intracellular oxygen generation as previously described, sharing several other characteristics with “Ca. Methylomirabilis lanthanidiphila” (Table 1). Finally, several previously identified and novel putative sulfide oxidizers were identified based on key gene analyses (Figure 4 and 5): MAG 58 Sulfuricellaceae, MAG 10 Anderseniellaceae, MAG 23 Dokdonella, MAG 40 Notoacmeibacter, MAG 50 Rhodocyclaceae 1, MAG 52 Rhodocyclaceae 3, MAG 57 Sulfuricella denitrificans, MAG 56 Sulfuricella, MAG 64 Thiobacillus, MAG 65 Thiohalobacteraceae, and MAG 63 Thermomonas.
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