156 Chapter 5 Table 3. “Nitrite and sulfite reductase 4Fe-4S domain containing protein” (PFAM: PF01077.27) transcript expression. Transcripts that showed Padj<0.05 for at least one condition were included for the analysis and indicated in bold. Genes were classified based on their KEGG number or INTERPRO id (for sulfite reductases): adsrA (K11180) & dsrB (K11181); bnirB (K00362) & nirD (K00363); cassimilatory sulfite reductase (aSir) (IPR051329), aSir/Group I Dsr-LP (IPR045854) or Group III Dsr-LP (IPR045169, IPR045854). We indicated an asterisk for the gene found in the *unbinned fraction and/or when manually assigned to a MAG. Sulfite reductases were classified as in Yu et al. (2018). Transcripts were ordered from highest to lowest expression change from T0-T3. Condition differences (T0-T1, T0-T2 and T0-T3) indicate log2 fold chain (FC) values. “NA” (Not Available). adsrA/dsrB bnirB/nirD Sulfite reductase group MAG (contig) T0-T1 T0-T2 T0-T3 nirB Methylomirabilis_oxyfera (159_119) 0.24 6.91 8.65 aSir/Group I Dsr-LP *Methanoperedens (497_111) -0.38 4.98 5.26 aSir Hyphomicrobiaceae_g_AWTP1_13 (224_104) -2.89 2.01 5.00 aSir Methylomirabilis_oxyfera (583_20) 0.31 3.64 4.29 nirD Methylomirabilis_oxyfera (159_120) 0.58 4.47 3.98 Group III Dsr-LP *Methanoperedens (497_44) -1.63 2.08 2.86 Group III Dsr-LP Methanoperedens (836_64) -1.54 1.28 2.32 aSir *Methanoperedens (497_74) -1.12 1.32 1.72 dsrB Hyphomicrobiaceae_g_AWTP1_13 (1039_26) 1.41 1.66 0.08 dsrA Casimicrobiaceae_g_JACPUX01 (118_108) 2.11 0.81 -0.39 dsrA Hyphomicrobiaceae_g_AWTP1_13 (1039_25) 1.80 0.84 -0.43 dsrA Desulfobacterales_g_SpSt_501_1 (1804_13) 0.98 -1.39 -1.78 dsrA Ferrovibrionaceae_2 (132_95) 2.00 -0.14 -2.86 dsrB Rhodocyclaceae_g_JACRPB01 (1466_35) NA -5.30 -2.89
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