188 Chapter 6 ymonoxaT Bacteria “Ca. Methanoperedens” ANME 2a/3 ANME 2c Borg (kamA - ablB) Borg (only kamA) fam. Methanosarcinaceae order. Methanomassiliicoccales class. Methanomicrobia/Methanocellia genus. Methanosalsum/Methanohalophilus/ Methanococcoides Euryarchaeota other Archaea NZVIAQ01000011.1166 NZAZAJ01000001.1 1296 NZFNCA01000002.134 NZJAGGKD010000001.1 15 NZFOUJ01000002.1359 DAZF01000073.119 JADQBY010000028.174 NZMBKP01000006.171 PXAV01000040.113 NZCP058215.12499 DACO01000200.1 5 JAFGCK010000067.120 NZPGGK01000002.1 324 CP003083.11806 JADQBZ010000004.1 92 NZFOHQ01000006.179 NZCAAGSW010000003.1142 NC007955.1285 NZJRHO01000002.1 94 JAFGOO010000202.1 8 p__Cloacimonadota;c__Cloacimonadia;o__JGIOTU-2;f__JGIOTU-2;g__JGIOTU-2;s__JGIOTU-2sp016932035 Hyperhalophilic_Methanogens_g_Methanohalophilus_NZJAGGLK010000004.125 NZOBDR01000002.1240 NZQBKB01000002.11878 NC014002.11415 NZCP017921.11279 NZCP017881.1538 NZRDSK01000009.1233 NC015676.11049 PWGQ01000134.126 MCK5643319.1 HJH31704.1 HJH30302.1 MCL7411502.1 LADT01000068.1 14 JUEF01000049.1 47 p__Firmicutes_C;c__Negativicutes;o__Sporomusales;f__UBA7701;g__CTSoil-080;s__CTSoil-080 sp017889635 s__Methanoperedens_sp014859785.1 MBE0524164.1 JACUTQ010000075.1 2 TFH41657.1 SR-VP 0-2 scaffold 141 936180 80 SRVP18 hole-7m-from-trench 1 80cm scaf 35 ext 315-rev 6-w terminal repeats 289 292 RI LANI F32 d l o f f a cs m c04C9102 0162PV-RS-KCALB SR-VP 26 10 2019 2 100cm scaffold 1 240 MancosShaleSediment B 50 Aug scaffold 849964 10 SR-VP 26 10 2019 2 100cm scaffold 48 48 rifcsphigho2 12 scaffold 1730 10 SR-VP 26 10 2019 2 100cm scaffold 5 86 SR-VP 26 10 2019 2 100cm scaffold 28 158 SR-VP 4-6 scaffold 141 1846199 49 SR-VP 0-2 scaffold 141 5187255 50 SRVP18 trench 6 60cm scaffold 2519 7 SRVP18 trench 6 60cm scaffold 17 265 SR-VP 0-2 scaffold 141 5176630 44 LILAC RifSed csp1 19ft 2 test LILAC rev17 terminal repeats-final 323 SRVP18 trench 6 60cm scaf 214 117 86 FINAL 509 s__Methanoperedens_sp_BLZ1 NZ NTMG01000031.1 60 s__Methanoperedens_sp_BLZ2 RifSed csp1 19ft 2 scaffold 16179 8 3 42 56 3 d lof facs3 t f 61 1p sc deSf iR MBU4077020.1 1 . 5 97998810 ps_snede r eponah t eM __s 8321.20000010YIMJZN s nec ude r o r t i n_snede r ep onah t eM_ _ s 1 . 5 32 69881 0_ps_sn ed e r epo nah t eM__s 1 . 538620210ps_snede r eponah t eM__s 1 . 580683209ps_snede r epon ah t eM__s 4111.200000010JFPJAC 1 . 55 1933509 ps_sn ede r epo nah t e M__ s__TLAS _FE R s__Methanoperedens_sp026552975.1 s__Methanoperedens_sp003104905.1 p__Firmicutes_B;c__Desulfitobacteriia;o__Desulfitobacteriales;f__Desulfitobacteriaceae;g__Desulfosporosinus;s__Desulfosporosinus sp003513495 p__Firmicutes_D;c__Dethiobacteria;o__DTU022;f__UBA8154;g__DUNV01;s__DUNV01 sp016841605 p__Firmicutes_B;c__Desulfitobacteriia;o__Desulfitobacteriales;f__Syntrophobotulaceae;g__Gracilibacter;s__Gracilibacter sp001516055 p__Firmicutes_C;c__Negativicutes;o__Sporomusales_A;f__Acetonemaceae;g__Acetonema;s__Acetonema longum NZ KB899432.1 11 NC 014364.1 3966 p__Spirochaetota;c__Spirochaetia;o__DSM-16054;f__Sediminispirochaetaceae;g__DSBN01;s__DSBN01 sp011046055 p__Firmicutes_G;c__UBA4882;o__UBA8346;f__UBA8346;g__Hydrogenispora;s__Hydrogenispora ethanolica p__Spirochaetota;c__Spirochaetia;o__Treponematales;f__Treponemataceae;g__DUOS01;s__DUOS01 sp002070025 p__Fibrobacterota;c__Chitinivibrionia;o__Chitinivibrionales;f__Chitinispirillaceae;g__JAAYPC01;s__JAAYPC01 sp012519985 p__Spirochaetota;c__Spirochaetia;o__Treponematales;f__Treponemataceae;g__DUOS01;s__DUOS01 sp012838745 JAHDRN010000001.1 312 JAHDQW010000007.1 184 p__Spirochaetota;c__UBA4802;o__UBA4802;f__JAFGJZ01;g__JAFGRW01;s__JAFGRW01 sp016934935 p__Spirochaetota;c__Spirochaetia;o__JC444;f__Marispirochaetaceae;g__JAFGQL01;s__JAFGQL01 sp016935695 p__Elusimicrobiota;c__Elusimicrobia;o__Elusimicrobiales;f__UBA9959;g__UBA9959;s__UBA9959 sp001871115 p__Marinisomatota;c__AB16;o__AB16;f__46-47;g__TCS52;s__TCS52 sp001577215 MBN2412094.1 p__KSB1;c__UBA2214;o__UBA2214;f__Zgenome-0027;g__JAFGSB01;s__JAFGSB01 sp016934825 p__Spirochaetota;c__UBA6919;o__GWB1-36-13;f__GWB1-36-13;g__GWB1-36-13;s__GWB1-36-13 sp001829155 CAJHIS010000002.1 5 RXIJ01000003.1 3 RPGO01000001.1 24 RXIK01000146.1 23 KAF5415260.1 AAU83197.1 RLG20449.1 PIXW01000025.1 2 p__Fibrobacterota;c__Chitinivibrionia;o__Chitinivibrionales;f__WJKA01;g__WJLW01;s__WJLW01 sp014728295 NZ SMGQ01000017.1 62 NZ SMAL01000008.1 76 DSCS01000029.1 72 JAFGWL010000065.1 4 QNCR01000235.1 1 DKER01000081.1 6 HCL00599.1 DPVY01000318.1 4 MCD6441411.1 RZYC01000051.1 1 JABMPJ010000215.1 1 JAEIOF010000021.1 18 JACNKZ010000018.1 4 DINM01000152.1 65 DSDE01000060.1 6 JAFGRT010000034.1 5 NZ BCNY01000015.1 560 PGYH01000094.1 14 NC 009051.1 208 NZ JMIO01000085.1 9 NZ JXOJ01000001.1 22 DASQ01000031.1 18 JAENYE010000001.1 412 JAENZF010000113.1 2 JAHDQM010000020.1 7 DAXW01000005.1 44 DAEF01000182.1 4 DUGU01000160.1 5 JAAYEG010000014.1 44 NZ LHQS01000001.1 595 NZ JAGGMN010000001.1 1547 NZ CM001555.1 2253 NZ PGCL01000002.1 585 JAENZA010000005.1 36 LTBL01000001.1 28 DSBQ01000009.1 2 JAENYT010000006.1 46 DSDF01000038.1 7 NZ JAAAWJ010000062.1 3 LKUD01000041.1 32 NZ CM001436.1 1130 JADFDO010000004.1 196 MVZQ01000038.1 3 DADR01000004.1 75 JAAZNH010000267.1 6 PGYK01000001.1 279 PGYM01000001.1 987 PIZH01000004.1 10 NC 008942.1 351 NIZU01000003.1 22 LMVO01000034.1 32 DAWV01000002.1 98 DAQC01000006.1 277 DAWO01000053.1 13 JAHDSI010000040.1 5 DASP01000012.1 88 DANP01000007.1 19 JAAEET010000043.1 2 JAAYCY010000026.1 22 NZ QGMZ01000039.1 141 CP077107.1 2703 JAFGOM010000112.1 12 NC 017034.1 930 NC 013665.1 1956 MVRA01000185.1 31 DARA01000021.1 8 QZAC01000022.1 9 QZAD01000012.1 4 DAJQ01000027.1 53 DUKV01000008.1 3 NZ JWHL01000008.1 62 SLSK01000122.1 7 SLQH01000474.1 9 JAFGKF010000116.1 11 PWZG01000400.1 1 PXDI01000308.1 3 SLKS01000310.1 6 JAFGEP010000166.1 4 PUNC01000133.1 80 PWXJ01000179.1 10 PWTM01000251.1 11 PWNM01000039.19 MHBK01000069.1 5 PWMW01000179.18 SLOZ01000075.1 4 PUOC01000011.1 18 NC002936.3 218 NZCP013074.1 339 NZCP006951.1170 DKFF01000013.19 NC014216.1 564 DSDS01000142.1 28 DIKC01000186.12 JAHJAA010000013.193 SLSR01000280.111 WJJU01000117.16 PWNH01000095.13 SKHQ01000016.112 SKGM01000051.15 PUJZ01000004.111 PWHV01000035.136 SKMN01000012.163 SLCP01000190.15 SKKZ01000114.1 3 DDWT01000012.160 NZFTMS01000010.132 NZKB891758.133 JAFGXN010000084.1 8 DIFF01000011.147 DLGE01000001.111 SLMV01000170.16 JAAZAW010000301.19 WJJK01000379.16 JAGXLH010000071.15 NC014507.11459 NZAXDV01000005.112 JAFGKW010000001.117 NZJAGGMM010000002.1 268 JAHJCU010000005.156 JAGYNW010000001.110 PWNJ01000120.128 PXBT01000028.13 JAFGWI010000194.11 NZ JWTM01000424.1728 NZ MWQY01000028.1 12 JAABTP010000136.1 7 MTKO01000119.1 18 RPGP01000029.1 394 JAAZAI010000534.1 5 JAFGXL010000030.1 44 NC 021182.1 3635 NZ BAEV01000002.1 29 NZ BOPZ01000002.1 26 NZ WHNX01000013.1 16 MFRJ01000090.1 3 DNRP01000133.1 7 NC 009975.1 1818 NC 009135.1 675 NC 009637.1 114 NZ CP026606.1 135 NC 009634.1 1258 NZ AQXV01000054.1 126 NC 009635.1 250 NZ CP029758.2 3744 121.930000010CGEEAJZN 821.03000010PCKQ 2061 . 336 410 CN 721 . 441000010NQ GFAJ 211.260000010TWGFAJ 91.060000010QXGFAJ NZ FSRH01000011.1 15 63511.366110PCZN NZ KE384518.1 246 JAAYSX010000036.1 7 DUUM01000119.1 39 NZ LRVM01000004.1 262 JACERV010000004.1 119 NZ FUWV01000013.1 39 NZ BDQY01000014.1 59 JAHDQS010000005.1 151 JAHDSQ010000011.1 102 NZ JJOY01000049.1 180 NZ CP009503.1 445 NZ CP009515.1 602 NZ CP009506.1 3544 NC 003552.1 4189 NZ CP009512.1 390 Plugger2003 abl1 WP 011032884.1 NZ CP009516.1 485 NZ CP009520.1 3431 NC 007355.1 711 NZ CP009528.1 3529 JAENZL010000059.1 9 NZ CP009505.1 473 DUKH01000147.1 14 JAENYU010000002.1 68 QKAP01000009.1 5 NZ FNQN01000005.1 214 NZ FNAQ01000003.1 148 PKUI01000189.1 13 PKTY01000370.1 1 NZ AAEW02000014.1 2 JAABXR010000365.1 5 SRVP18 trench 6 60cm scaf 214 117 86 FINAL 486 SR-VP 0-2 scaffold 141 5176630 61 LILAC RifSed csp1 19ft 2 test LILAC rev17 terminal repeats-final 306 SRVP18 trench 6 60cm scaffold 17 246 SR-VP 26 10 2019 2 100cm scaffold 488 52 SR-VP 26 10 2019 2 100cm scaffold 1 356 SR-VP 26 10 2019 2 100cm scaffold 5 258 s__Methanoperedens_sp026552975.1 RifSed csp1 16ft 2 scaffold 64955 1 164482dloffacs2tf911pscdeSfiR SRVP18 hole-7m-from-trench 1 80cm scaf 35 ext 315-rev 6-w terminal repeats 192 DFZT01000014.1 43 DOTD01000039.1 19 p__Firmicutes_B;c__Syntrophomonadia;o__Syntrophomonadales;f__Syntrophomonadaceae;g__Syntrophomonas_A;s__Syntrophomonas_A RifSed csp2 16ft 3 scaffold 737 63 QKAP01000009.1 8 PKUI01000189.1 15 PKTY01000370.1 2 DAQC01000006.1 278 JAABRU010000105.1 2 DSBL01000077.1 17 LILACRifSedcsp1 19ft 2testLILAC rev17 terminal repeats-final 324 SRVP18trench660cm scaf 21411786FINAL510 SR-VP0-2scaffold 141518725549 SR-VP4-6scaffold141184619948 SRVP18trench660cmscaffold25198 SRVP18trench660cmscaffold17266 SR-VP0-2 scaffold141517663043 SR-VP26 1020192100cmscaffold28159 SR-VP261020192100cmscaffold585 SR-VP261020192100cmscaffold4847 SR-VP261020192100cmscaffold1 238 BLACK-SR-VP26102019C40cmscaffold 23FINALIR 293 SRVP18hole-7m-from-trench1 80cmscaf 35ext 315-rev6-wterminal repeats290 SR-VP0-2scaffold141 93618081 CAJPFJ010000002.1115 REF_SALT__s__Methanoperedens_sp905339155.1 1mg RUfwitaliandom methanosarcinales1gMethanoperedenscontig1127778 s__Methanoperedens_sp026552975.1 s__Methanoperedens__sp014859785.1_JACUTP010000101.11 MBE0522021.1 CABMIA010000113.16 VVB87398.1 s__Methanoperedens_sp902386085.1 NZ NTMG01000031.161 1 mg RUfwitalian minmethanosarcinales 2Methanoperedenssp002487355contig199606 9RUfwkidraghoebarsingtwenteblz2GCF002487355.1 Methanoperedenssp002487355 NTMG01000031.161 WP 097298154.1 2 mg RUbrackishredsea GCA019912155.1 Methanoperedenssp002487355contig19862213 31125812gitnoc553784200pssnedereponahteM1selanicrasonahtemredlopjioowfURgm3 12 RUfwbes ettwigg2009ooijpolderGCA 008933945.1 Methanoperedenssp002487355WBTY01000002.171 NZJMIY01000002.1239 22UQParksetal17undefinedGCA002496325.1Methanoperedensnitroreducens.fna DAJM01000072.1 45 11UQfwnitrateammoniumGCF000685155.1MethanoperedensnitroreducensJMIY01000002.1 239 s__Methanoperedens_nitroreducens WP 241763308.1 KCZ72594.1 MBU4077019.1 JAHIHJ010000309.13 1EPFLsulfategrounw GCA 022013355.1 MethanoperedensN1.45702.6 JAKLQM010000219.1 1 1EPFL sulfategrounwGCA 022013355.1 MethanoperedensN1.45702.6 JAKLQM010000456.14 JAHIFK010000167.12 s__Methanoperedens_sp018900975.1 2 CALTECH mudvolcanoGCA 020793555.1ANME3 DQIO01000322.118 HJH30301.1 HJH31705.1 MCL7411503.1 ANME2A_JACUTQ010000075.1 1 MBE0524163.1 NC 015676.11050 g_Haloalkaliphilic_Methanogens_g_Methanosalsum_WP013898337.1 g_Haloalkaliphilic_Methanogens_g_Methanosalsum_PWGQ01000134.1 27 p__Firmicutes_D;c__Dethiobacteria;o__DTU022;f__UBA8154;g__DUNV01;s__DUNV01sp016841605 p__Firmicutes_B;c__Desulfitobacteriia;o__Desulfitobacteriales;f__Desulfitobacteriaceae;g__Desulfosporosinus;s__Desulfosporosinus sp003513495 p__Firmicutes_B;c__Desulfitobacteriia;o__Desulfitobacteriales;f__Syntrophobotulaceae;g__Gracilibacter;s__Gracilibacter sp001516055 KUO61932.1 p__Firmicutes_C;c__Negativicutes;o__Sporomusales_A;f__Acetonemaceae;g__Acetonema;s__Acetonema longum p__Firmicutes; c__Clostridia; o__Eubacteriales; f__Desulfotomaculaceae; g__Pelotomaculum; s__Pelotomaculum sp. p__Firmicutes_B;c__Peptococcia;o__Peptococcales;f__BICA1-8;g__BICA1-8;s__BICA1-8 sp000802045 KJS86988.1 p__Firmicutes_B;c__Peptococcia;o__Peptococcales;f__BICA1-8;g__BICA1-8;s__BICA1-8 sp000961765 KJS19952.1 p__Firmicutes_C;c__Negativicutes;o__Sporomusales;f__UBA7701;g__CTSoil-080;s__CTSoil-080 sp017889635 NZ CP029758.2 3745 NZJAEEGC010000039.1 22 p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium_AF;s__Clostridium_AF grantii p__Marinisomatota;c__UBA2242;o__UBA2242;f__UBA2242;g__UBA9312;s__UBA9312 sp003527965 DADR01000004.174 PGYM01000001.1 986 DAPW01000033.1 29 PGYK01000001.1278 JAAZNH010000267.1 5 PIZH01000004.19 DAWV01000002.1 99 MVZQ01000038.1 4 JADFDO010000004.1 197 DAQC01000006.1276 NIZU01000003.123 NC008942.1 350 LMVO01000034.1 31 JAAYCY010000026.1 21 NZ QGMZ01000039.1 142 CP077107.1 2702 JAFGOM010000112.1 13 DUGV01000277.1 2 13111.634100MCZN 06411.705410CN 311.50000010VDXAZN 811.100000010WKGFAJ 9621.200000010MMGGAJZN 4311.606620PCZN 5111.736900CN 71811.579900CN 6761.531900CN 75211.436900CN 1521.536900CN 5211.45000010VXQAZN 517134200ps9285ABU__s;9285ABU__g;9285ABU__f;9285ABU__o;9285ABU__c;atodanomitamrA__p;airetcaB__d 517134200ps9285ABU__s;9285ABU__g;9285ABU__f;9285ABU__o;9285ABU__c;atodanomitamrA__p;airetcaB__d 528755300ps10MNWP__s;10MNWP__g;10BHLS__f;selaitnedenegordyH__o;aitnedenegordyH__c;atotnedenegordyH__p;airetcaB__d 586278100ps7748ABU__s;7748ABU__g;7748ABU__f;7748ABU__o;7748ABU__c;atotamosiniraM__p;airetcaB__d 81.630000010XSYAAJ 831.91100010MUUD NZ CP011663.1 1535 NZ KE384518.1 247 NZ LRVM01000004.1 263 JACERV010000004.1 118 NZ FSRH01000011.1 16 NZ JAEAGR010000002.1 91 JAHDLO010000047.1 9 NZ FUWV01000013.1 38 NZ RJLQ01000003.1 117 JAAYPF010000098.1 16 RZYX01000024.1 9 NZ QRCT01000012.1 28 PHAG01000002.1 70 PGZO01000015.1 64 NZ LGSS01000006.1 90 NC 021182.1 3634 NZ BAEV01000002.1 28 NZ BOPZ01000002.1 27 NZ WHNX01000013.1 15 MFRJ01000090.1 4 DNRP01000133.1 6 d__Bacteria_p_Firmicutes_c_Clostridia_o__Peptostreptococcales_NZ BDQY01000014.1 58 NZ VIAQ01000011.1 165 NZ AZAJ01000001.1 1295 NZ FNCA01000002.1 35 NZ FOUJ01000002.1 358 NZ JAGGKD010000001.1 16 PXAV01000040.1 12 NZ MBKP01000006.1 70 JADQBY010000028.1 73 DAZF01000073.1 18 DACO01000200.1 4 NZ CP058215.1 2498 NZ PGGK01000002.1 323 JAFGCK010000067.1 21 CP003083.1 1807 JADQBZ010000004.1 93 JAHDQW010000007.1 183 JAHDRN010000001.1 313 d__Bacteria_p__Spirochaetota_JAFGWI010000194.1 2 p__Bdellovibrionota; c__JAFGOL01; o__JAFGOL01; f__JAFGOL01; g__JAFGOL01; s__JAFGOL01 sp016926495 NZ JRHO01000002.1 93 5 KAUST submarcanSC GCA000765475.1 Methanococcoides methylutens JRHO01000002.1 93 7 CDF mediterranean GCA 900774285.1 Methanococcoides NM1 CAAGTW010000001.1 382 7 CDF mediterranean GCA 900774285.1 Methanococcoides NM1 CAAGTW010000001.1 377 JAFGOO010000202.1 7 NZ FOHQ01000006.1 78 7 CDF mediterranean GCA900774285.1 Methanococcoides NM1 CAAGTW010000004.1 79 NZ CAAGSW010000003.1 143 6 CDF baltic GCA 900774055.1 Methanococcoides AM1 CAAGSW010000003.1 146 NC 007955.1 284 JAFGDR010000005.1 2 NZ JAGGLK010000004.1 26 NC 014002.1 1414 NZ QBKB01000002.1 1879 NZ CP017921.1 1280 NZ CP017881.1 539 NZ OBDR01000002.1 239 NZ RDSK01000009.1 232 PKTM01000015.1 4 NC 013943.1 866 PKTN01000004.1 97 WTXA01000001.1 652 NZ SMGG01000004.1 657 DKFP01000004.1 355 MIAO01000003.1 17 DDQW01000036.1 15 DGAD01000054.1 4 JAAYBI010000052.1 4 DUPM01000009.1 5 SKKY01000190.1 6 RZYW01000008.1 9 NC 014633.1 602 QKCP01000030.1 28 NZ CP071444.1 925 NZ JADNRK010000004.1 95 JAEINS010000010.1 51 JAABXR010000365.1 2 NZ FONW01000016.1 8 NZ LGIA01000175.1 24 NZ WVEM01000003.1 202 JAEUSS010000003.1 5 NZ WKLH01000001.1 1030 NZ FONA01000010.1 83 JAEINY010000012.1 11 JAFGXN010000084.1 9 NZ CP030040.1 1021 JAHIQT010000213.1 3 PGZR01000060.1 9 NC 016894.1 391 NZ LGYO01000022.1 39 NZ RXYA01000002.1 196 JAENWK010000051.1 7 NZ FOYZ01000008.1 51 WJME01000001.1 10 DIFF01000011.1 48 DFBJ01000009.1 490 NJER01000004.1 84 NC 016620.1 856 PCUW01000056.1 109 PBCC01000016.1 33 JAAYJM010000035.1 4 NZ SMAL01000008.1 75 NZ SMGQ01000017.1 61 WJIP01000341.1 12 JAAYCB010000504.1 17 MWAO01000269.1 4 DLCK01000084.1 46 PAIY01000083.1 5 JAAZAW010000301.1 12 CAJHIS010000002.1 4 RXIJ01000003.1 4 RPGO01000001.1 25 DTYY01000036.1 2 RXIK01000146.1 24 QMVM01000323.1 4 DAWR01000019.1 4 PIXW01000025.1 3 SLLR01000030.1 8 PWVL01000047.1 2 LLYT01000004.1 13 QKAP01000009.1 3 NZ FNQN01000005.1 213 JAAXQE010000045.1 1 NZ JOMG01000002.1 2000 JAFGXV010000028.1 21 7411.30000010QANFZN JAFGAM010000031.1 6 JAGYPB010000033.1 15 111 1 . 80000 0010NUWCA J ZN SLDV01000123.1 2 PWNJ01000120.127 1571.100000010EQFFAJZN SLMV01000170.1 5 DSDS01000142.129 NC 014216.1563 DIKC01000186.1 3 JAHJAA010000013.194 DUZU01000073.1 2 NC 002936.3218 NZ CP013074.1339 NZCP006951.1 170 DKFF01000013.1 10 JABZFP010000013.1114 CAFBRK010000120.19 LJNK01000043.19 JABZFO010000150.1 4 NZ AP021874.11758 WJLW01000015.1 131 JAABSP010000046.129 JAABRU010000105.1 3 NZMWQY01000028.111 NZJWTM01000424.1727 NZJMIO01000085.110 NZJXOJ01000001.123 NC009051.1209 NZBCNY01000015.1561 PGYH01000094.115 DASQ01000031.117 DAFZ01000027.18 JAENZF010000113.13 DAXW01000005.145 JAHDQM010000020.16 JAENYE010000001.1411 DAEF01000182.13 DUGU01000160.14 JAAYEG010000014.143 DAXW01000003.148 NZ FMID01000031.1 36 DAYL01000022.1 2 JAAZGM010000090.110 JAGVUK010000090.13 DANY01000033.1 55 DAFR01000016.110 JAAZID010000390.1 2 JAAYND010000103.15 JAAZOW010000286.13 NC018227.2220 LFRN01000145.111 NZLHQS01000001.1 596 NC009464.1169 MVRA01000185.130 NC013665.11955 NC017034.1929 NZCM001555.12252 NZPGCL01000002.1586 NZJAGGMN010000001.1 1546 JAENZA010000005.137 DSBQ01000009.13 LTBL01000001.1 29 NZBBBG01000004.1258 JAAYIO010000097.14 NZ JAAAWJ010000062.14 LKUD01000041.1 33 JAENYT010000006.1 47 DSDF01000038.16 DUKV01000008.1 2 DAJQ01000027.1 52 QZAC01000022.1 10 QZAD01000012.13 SLSK01000122.1 8 NZ JWHL01000008.161 NZKB891758.132 NZ FTMS01000010.131 JAAEES010000197.1 1 DANP01000007.118 JAAEET010000043.11 DAWO01000053.114 LKUF01000495.1 2 JAHDSI010000040.1 4 DASP01000012.1 89 DARA01000021.17 PWGE01000171.14 JACZKF010000207.13 SLID01000067.118 WJMG01000063.119 JAABSQ010000059.1 7 NC 013223.1 699 NZ JAEX01000002.1205 DBFQ01000075.1 4 JAGXKT010000007.116 NZ JPIK01000012.1 153 NZ PZKN01000023.1 16 NZ KI912608.1 2428 NZ JYNO01000001.1 438 REDL01000059.1 11 NZFMXO01000021.12 DSBL01000077.1 14 NZ FUYC01000025.1 9 NZ ATVA01000014.1 272 PWPY01000047.1 6 NZJMKT01000011.1 30 NZ KN882175.1 241 JACCQO010000003.144 61.42000010ZLUAZN 4311.13000010IHTAZN 731.53000010VHWP 211.40000010ZJUP 41.15000010MGKS 41.59000010HNWP 311.61000010QHKS 1411.594915IKZN 29361.578120PAZN 41.09100010PCLS 461.21000010NMKS 41.41100010ZKKS 571.45000010MWDD 951.21000010TWDD 21.70000010BLMD DSBX01000252.1 3 NZ AZAC01000001.1 98 NZ SMDD01000001.1 1196 NZ PVTZ01000011.1 74 NZ JAECVR010000006.1 72 NZ FOCQ01000003.1 218 NZ SMAG01000002.1 395 NZ JACXAG020000006.1 93 NZ JPST01000012.1 58 NZ REFP01000009.1 381 NZ JACEOL010000025.1 67 NZ LN812102.1 362 NZ BMHQ01000001.1 54 NZ ACJM01000015.1 20 DLMZ01000041.1 38 NZ CP009520.1 3430 NC 007355.1 712 NZ CP009528.1 3528 NZ LMVP01000301.1 32 NZ CP009503.1 446 NZ JJOY01000049.1 181 NZ CP009515.1 603 NZ CP009516.1 484 NC 003552.1 4188 NZ CP009506.1 3543 NZ CP009512.1 389 Plugger2003 abl2 WP 011032885.1 JAENZL010000059.1 8 DUKH01000147.1 13 JAENYU010000002.1 67 NZ CP009505.1 474 JAFGQN010000144.1 26 JAFGWT010000062.1 13 JAFGXQ010000060.1 10 NZ CP021023.1 1850 NZ CP019633.1 1535 SLOZ01000075.1 3 PWMW01000179.1 7 PUOC01000011.1 19 JAABTM010000108.1 13 JAABTD010000059.1 3 NZ JAFFQE010000013.1 18 PIVY01000029.1 3 WJIQ01000110.1 5 PXBT01000028.1 4 JAFGWL010000065.1 5 DSCS01000029.1 71 MHBK01000069.1 6 NZ JAAEEH010000004.1 67 JAAYFZ010000286.1 4 NZ NIJK01000040.1 18 NZ NIJM01000016.1 60 NZ CP068230.1 1168 NZ NGVS01000033.1 17 NZ AUGL01000002.1 247 NZ SWCI01000005.1 53 NZ FQXG01000002.1 491 NC 014541.1 1216 NZ KB906979.1 25 JABXKR010000129.1 8 NZ AUAM01000067.1 9 NC 009831.1 1874 NZ RXNU01000022.1 48 NZ RXNV01000021.1 1 NZ QRCS01000015.1 41 QZLB01000240.1 10 NZ WIAA01000001.1 861 NZ PNEC01000021.1 122 NZ PPSW01000012.1 38 NZ CP013187.1 1229 NZ LKBD01000034.1 10 NZ CP009889.1 303 NZ CP011040.1 254 AT A B C LAM “Ca. Ethanoperedens” Sup. Fig. 10A Sup. Fig. 10B Fig. 3C Reference Ambiguous alignment Putative fusion protein LAM-AT This study LILAC RifSed csp1 19ft 2 test LILAC rev17 terminal repeats-final 324 SRVP18 trench 6 60cm scaf 214 117 86 FINAL 510 SR-VP 0-2 scaffold 141 5187255 49 SR-VP 4-6 scaffold 141 1846199 48 SRVP18 trench 6 60cm scaffold 2519 8 SRVP18 trench 6 60cm scaffold 17 266 SR-VP 0-2 scaffold 141 5176630 43 SR-VP 26 10 2019 2 100cm scaffold 28 159 SR-VP 26 10 2019 2 100cm scaffold 5 85 SR-VP 26 10 2019 2 100cm scaffold 48 47 SR-VP 26 10 2019 2 100cm scaffold 1 238 BLACK-SR-VP 26 10 2019 C 40cm scaffold 23 FINAL IR 293 SRVP18 hole-7m-from-trench 1 80cm scaf 35 ext 315-rev 6-w terminal repeats 290 SR-VP 0-2 scaffold 141 936180 81 s__Methanoperedens_sp026552975.1 s__Methanoperedens_nitroreducens_Vercelli_S1_sp905339155.1 s__Methanoperedens_sp902386085.1 s__Methanoperedens_nitroreducens s__Methanoperedens_sp018900975.1 ANME2A p__Firmicutes; s__DUNV01 sp016841605 p__Firmicutes; s__Desulfosporosinus sp003513495 p__Firmicutes; s__Gracilibacter sp001516055 p__Firmicutes; s__Acetonema longum p__Firmicutes; s__Pelotomaculum sp. p__Firmicutes; s__CTSoil-080 sp017889635 p__Marinisomatota; s__UBA9312 sp003527965 p__Firmicutes; s__Clostridium_AF grantii p__Armatimonadota; s__UBA5829 sp002431715 p__Armatimonadota; s__UBA5829 sp002431715 p__Hydrogenedentota; s__PWNM01 sp003557825 p__Marinisomatota; s__UBA8477 sp001872685 p__Bdellovibrionota; s__JAFGOL01 sp016926495 Methanogens_f__Methanosarcinaceae p__Firmicutes; s__BICA1-8 ANME3 s__Methanoperedens_sp022013355.1 s__Methanoperedens_sp_BLZ2 Haloalkaliphilic_Methanogens_g__Methanosalsum p_Cyanobacteria p_Firmicutes; o_Clostridiales Methanogens_c_Methanomicrobia Methanogens_Euryarchaeota Ambiguous alignment This study ablB expression in MAG detected plasmid-associated genome <70 70-79 80-88 91-95 98-100 Bootstrap values 1 brackish freshwater ablB expression - salinity source Figure 3. Lysine 2,3-aminomutase (LAM) and β-lysine N6-acetyl transferase (AT) amino acid tree. A Taxonomical color-coded overview of LAM and B AT gene tree with a particular focus on ANME, methanogens and Borg encoded sequences. Putative fusion proteins refer to contigs containing double-annotated kamA and ablb genes whereas ambiguous alignment indicate sequences that did not surpass the homogeneity character composition established by Iqtree. C Zoomed in view on “Ca. Methanoperedens” neighboring sequences in the AT tree in panel B (indicated with black lines). Bootstrap support values for 1,000 replicates analysis. Branch length depict average number of amino acid substitutions per site. See Supplementary Fig. 10 for zoomed in version of “Ca. Methanoperedens” neighbouring sequences in
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