200 Chapter 6 microbiomes and resulting competitive pressure should also be addressed. Hence, future research integrating field studies with more complex laboratory experimental designs are needed. Data Availability Raw 16S rRNA gene sequences and metagenomics/transcriptomics reads have been uploaded to the European Nucleotide Archive with project number PRJEB70247. Proteomics raw data, reference sequence database and database search files have been deposited in the ProteomeXchange consortium database with the dataset identifier PXD048239. Microscopic experiments can be accessed via the FigShare project link: https://figshare.com/s/1f0f21a456665259077c Author contribution MJEM, MSMJ, ISA and CUW planned and designed the study. MJEM executed the research project. AL, SJM, GWT provided omics analysis expertise and support. MJEM and RJ conducted metabolomics analysis. JVE and MP extracted proteins and applied statistical analysis on both the metaproteome and sialic acids measurements. YL extracted and measured sialic acids and sialidase activity. MJEM wrote the manuscript draft with contributions from all authors. All authors reviewed and approved the final version submitted. Acknowledgements We thank Rob de Graaf for metabolomics sampling and PHA quantification assistance. We also express our gratitude to Theo van Alen for general sequencing support and Jelle Postma for confocal microscopy assistance and microscopy analysis discussion. We acknowledge the expertise of Jan Zethof for the analysis of rt-qPCR data. Funding This study was supported by the SIAM Gravitation grant funded by NWO [Grant number 024.002.002]. MJEM was furthermore supported by the ERC Synergy Grant MARIX [Grant number 854088]. SJM was supported by a Future Fellowship from the Australian Research Council (FT190100211). CUW was supported by an NWOVIDI Talent Grant [Grant number VI.Vidi.223.012].
RkJQdWJsaXNoZXIy MTk4NDMw