41 Introduction and thesis outline Parallel to Chapter 5, in Chapter 6, we also exploited a “Ca. Methanoperedens” spp. culture as study model to investigate a different physiological stressor: increased salinity. Complementary methane oxidation activity assays, visualization techniques, PHAs quantification, extracellular polymeric substance measurements together with meta-omics analyses and targeted molecular techniques were leveraged for a holistic view on “Ca. Methanoperedens” marine salinity thresholds and acclimation time as well as active adaptation mechanisms. Chapter 7 dived into the unique chemistry of meromictic Lake Cadagno to reveal the potential for “Ca. Methanoperedens´ spp.” representatives to engage in syntrophic partnerships with SRB. In situ biogeochemistry, enrichments with different electron acceptors, methane oxidation activity assays and metagenomics were merged to explore the unique features that could sustain this S-AOM enabling interaction. Finally in Chapter 8, we integrate all key research outcomes of this thesis and discuss methodological biases, its current implications and applications on the field, as well as provide an overview of future perspectives. 1
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