87 Contrasting methane, sulfide and nitrogen regimes in coastal sediment bioreactors −0.5 0.0 0.5 1.0 1.5 −2 −1 0 1 NMDS1 NMDS2 Alpha diversity measure A B 2000 1000 500 1500 0 5 10 15 Time (months) Gammaproteobacteria eu. eu. bf. oli. oli. bf. 2 3 5 6 7 8 9 10 11 12 14.5 15.5 Campylobacterota Other Chloroflexi Actinobacteriota Desulfobacterota Acidobacteriota Unclassfied Plantomycetota Calditrichota Bacteroidota Alphaprotebacteria Relative bacterial abundance (%) 60 40 20 0 60 40 20 0 60 40 20 0 60 40 20 0 C 2 3 5 6 7 8 9 10 11 12 14.5 15.5 oli. eu. Bacteria - Chao Richness Figure 2. A Nonmetric multidimensional scaling (NMDS) based on Bray-Curtis Dissimilarity of 16S rRNA gene bacterial amplicon sequencing variants (ASVs) per bioreactor substrate loading. Dimensionality was set at k= 3 and recorded a stress value of 0.04571603. Filled circle, inoculum; black outlined circle, month 2 sample; circles, planktonic samples; squares, biofilm samples; pink symbols, oligotrophic bioreactor; green symbols, eutrophic bioreactor. B Bacterial 16S rRNA gene ASVs richness index was calculated via Chao index and indicated as Alpha diversity measure (y-axis) per sample source across time (x-axis). Black circle, inoculum; circles, planktonic samples; squares, biofilm samples; pink symbols, oligotrophic bioreactor; green symbols, eutrophic bioreactor. Panel C represents from top to bottom top 5% bacterial 16S rRNA gene ASVs reads recovered at phylum level for all time points and substrate loading conditions. Samples were ordered from eutrophic (eu.) to oligotrophic (oli.), including biofilm (bf.) from month 6 onwards. 3
RkJQdWJsaXNoZXIy MTk4NDMw