89 Contrasting methane, sulfide and nitrogen regimes in coastal sediment bioreactors 11 onwards (excluding the biofilm) despite being a system saturated in methane and excess nitrate (Supplementary Figure 5 and 7 and Supplementary Table 3). The methane-oxidizing microbial community was intricately linked with the nitrogen cycling in the bioreactors. It seemed that DNRA observed in the oligotrophic bioreactor was primarily performed by “Ca. Methanoperedens BLZ2” sp. (Figure 3B) whereas the assimilatory nirB was more widely distributed among different MOB (Supplementary Figure 8). Although denitrification potential was encoded in several Methylomonas genomes in the eutrophic system (Figure 4A, Supplementary Figure 8 and Supplementary Table 4), it was clear that non-methanotrophic MAGs had a much larger contribution to denitrification, largely sulfide oxidizers including Thiobacillus and Sulfurimonas in the eutrophic bioreactor and Magnetovibrio, Thiomonas, and Thiothrix in the oligotrophic reactor (Supplementary Figure 8, Supplementary Table 1 and 5). B A Figure 3. A Total bacterial Amplicon Sequencing Variants (ASVs) recovered for all methanotrophic families across time and conditions: eu(trophic) or, ol(igotrophic) and with/without biofilm (bf.) B Percentage of top (> 10%) methanotrophic genome contribution to functional marker gene coverage for dissimilatory nitrate reduction to ammonium (DNRA): nrfA 3
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