90 Chapter 3 Oligotrophic bioreactor’s microaerophilic and sulfide-tolerant methanotrophic community members were metabolically more diverse and unexplored To get a better understanding of C, N, and S cycling potential of the methanotrophic members of the communities, we investigated 12 high quality MOB MAGs, and then clustered them based on abundance patterns (Figure 4A, Supplementary Figure 5 and Supplementary Table 4). More than half of these MAGs belonged to the Methylomonadaceae family, with Methylomonas, Methylovulum, Methylobacter, and subgroup Methylomonadaceae KS41 as genera. While the MAGs Methylovulum and Methylomonadaceae subgroup KS41 were more abundant in the oligotrophic system, Methylomonas and Methylobacter got more enriched in the eutrophic system (Figure 4A and Supplementary Figure 5). Based on average amino acid identity (AAI), all Methylomonadacae MAGs, except for the ones labeled “Methylomonas whittenburyi” and Methylomonas 4, may represent new species (Supplementary Figure 9 and 10). All MOB MAGs encoded for soluble or particulate methane monooxygenase (sMMO or pMMO) together with xoxF encoded lanthanide-dependent methanol dehydrogenases (MDHs, Figure 4A). Some MAGs also encoded the calcium dependent MDHs (Figure 4A). Many of the MOB MAGs seemed capable to detoxify sulfide via sqr, or included sox genes subunits (Figure 4A-B).
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