91 Contrasting methane, sulfide and nitrogen regimes in coastal sediment bioreactors Figure 4. A Functional annotation of high quality (>86% complete and <8% contaminated) metagenome assembled genomes (MAGs) of methanotrophs in two bioreactor systems. MAGs were clustered based on Z-score mean normalized coverage patterns depicted using a heatmap with individual rows depicting the time of sampling (7, 14.5, 15.5 months) and location in bioreactor sample (eu., eutrophic; eu.bf., biofilm eutrophic; ol., oligotrophic; ol.bf., oligotrophic biofilm). Functional marker genes are depicted as presence (filled)/absence (empty) matrix with color coding for metabolism. Purple, methane metabolism; light blue, oxidases; dark blue, nitrogen metabolism; orange, sulfur metabolism. Marker genes encoding for (part) of the following enzymes were queried against the MAGs: mcrABC, methyl coenzyme M reductase; pmoA, particulate methane monooxygenase; mmoX, soluble methane monooxygenase; mxaF, Ca-dependent methanol dehydrogenase; xoxF, La-dependent methanol dehydrogenase; cydAB, cytochrome bd oxidase; coxAB, cytochrome c oxidase; haoA, hydroxylamine dehydrogenase; nrfA, nitrite:ammonium oxidoreductase; narG, nitrate reductase; napA, nitrate reductase (periplasmic); nirK/nirS, nitrite reductase; norB, nitric oxide reductase; nosZ, nitrous oxide reductase; nifH, nitrogenase; sqr, sulfide:quinone oxidoreductase; sat, sulfate adenylyl transferase; soxAXYZ, sulfur oxidation system. B Percentage methanotrophic MAG contribution to the sulfide quinone oxidoreductase (sqr) gene coverage (>1%) during the three sampled months (7, 14.5, 15.5) and in the different bioreactor samples (eu., eutrophic; eu.bf., biofilm eutrophic; ol., oligotrophic; ol.bf., oligotrophic biofilm). Color coding indicates which MAGs contributed more to the sample’s sqr gene coverage. MAGs that harbored two gene copies were indicated with and without dot filling. MAGs were classified to the lowest known GTBD-Tk taxonomical category. Others category (grey) refer to the rest of non-methanotrophic MAGs. 3
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