cxxvii 10C. Local protocol for embryo quality assessment Table 10.S3 Local Embryo Quality Assessment Protocol: Embryo quality is based on the appearance of the embryos on day 1, 2 and 3. A final embryo quality assessment is given on day 3 Day Criterion Super Good Fair Moderate Poor 1 Type pn* 2pnª 2pnb /0pn à 2pnª 2pnb/0pn à 2pnª 2pnª / 2pnb / 0pn/ 1pn with IVF 2pnª / 2pnb / 0pn / 1pn No vacuoles Some small vacuoles Some small vacuoles N.a. N.a. 2 Number of Cells 4 Cells 4/5 Cells 2-5 Cells ≥2 Cells ≥ 2 Cells Fragmentation ≤20% (score 1+2*) ≤20% ( score1+2) ≤50% (score 1+2+3) ≤50% (score 1+2+3) N.a. Blastomere Uniformity Uniform Uniform/Somewh at uneven N.a. N.a. N.a. Mulitnuclear blastomeres (MNB) None None None MNB’s ≤25 % N.a. Vacuoles/Irregul arities None None Some vacuoles N.a. N.a. Clarity Clear Clear N.a. N.a. N.a. 3 Number of Cells 8/9 Cells 7-10 cells / starting. Morula 6-10 cells / starting Morula ≥ 4 cells ≥ 4 cells Fragmentation ≤20% (score1+2) ≤20% (score 1+2) ≤20% (score 1+2) ≤50% (score 1+2+3) N.a. Blastomere Uniformity Uniform/Somew hat uneven Uniform/Somewh at uneven N.a. N.a. N.a. Mulitnuclear blastomeres None None Some vacuoles N.a. N.a. Vacuoles/Irregul arities Clear Clear N.a. N.a. N.a. 2pnb à If the embryo has a lower 2pn score (a instead of b), the overall quality will decrease by one level. There should be progression between days 2 and 3, or there should be at least 2 blastomeres present for an embryo to have a quality score of ‘II’. If this is not the case, the embryo is automatically scored as having a quality of III.
RkJQdWJsaXNoZXIy MTk4NDMw