14800-DvRappard

96 Chapter 6 Figure 3. Robust microglia/macrophage activation and M2-like polarization in the white matter of transplanted MLD patients. (A-C) stain against the general macrophage marker CD68 shows similar abundance of immunopositive cells in transplanted (blue bars) and untreated patients (purple bars). (D-F) Stain against CD45, a marker for activated microglia/ macrophages, reveals a strong microglia cell activation in the white matter of transplanted compared to untreated patients (color bars as in A-C). (G,H and J,K) Stain against the M1 marker CD40 shows moremarkedM1-polarization of microglia/macrophages in the brains of untreated compared to transplanted patients, whereas stain against the M2-marker CD163 shows the opposite. (I) Quantification of M1- versus M2- versus double polarized cells demonstrates lower numbers of M1-polarized cells and greater numbers of M2- and double polarized cells in treated compared to untreated patients.