15502-m-pleumeekers

Primers and probes Human specific genes hsGAPDH Fw: AGCTCACTGGCATGGCCTTC Rev: CGCCTGCTTCACCACCTTCT hsACAN Fw: CAGCCACCACCTACAAACGCAG Rev: CTGGGTGGGATGCACGTCAGC Bovine specific genes bsGAPDH Fw: GTCAACGGATTTGGTCGTATTGGG Rev: TGCCATGGGTGGAATCATATTGG bsACAN Fw: GGACACTCCTTGCAATTTGAGAA Rev: CAGGGCATTGATCTCGTATCG Table 2. Sequences of primers and probes for qRT-PCR. GAPDH = GlycerAldehyde 3-Phosphate DeHydrogenase ; ACAN = Aggrecan ; hs = human-specific ; bs = bovine- specific. 114 CHAPTER 6