15502-m-pleumeekers

Figure 4. Cartilage matrix formation in constructs containing h BMSCs and/or chondrocytes, 8 weeks after subcutaneous implantation in mice. Biochemical evaluation of the sGAG (A) and collagen (B) content, 8 weeks after subcutaneous implantation. The left graphs demonstrate the amount of matrix components per construct, whereas for the right graphs matrix production is normalized to the initial number of seeded PCs. Data are shown as box-whisker plots. For statistical evaluation, a one-way ANOVA was used followed by a Fisher's least significant difference post-hoc comparisons test . PC = Primary Chondrocyte ; h BMSC = human Bone-marrow-derived Mesenchymal Stem Cell ( n=2 experiments with 2 different donors) ; b EC = bovine Ear Chondrocyte ( n=2 experiments with 3 pools of donors) ; b NC = bovine Nasal Chondrocyte ( n=2 experiments with 3 pools of donors). Per experiment, 2 samples were used for analyses. 119 CO-CULTURE: A PROMISING CELL-BASED THERAPY FOR FACIAL CARTILAGES 6