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time needed for the stress to decrease to half of its maximum value, was computed to estimate the viscoelastic relaxation after the first strain application, as described previously [329]. Statistical analysis Statistical analyses were performed with Statgraphics Centurion Version 17 (Statpoint Technologies, VA, USA). For cytotoxicity analysis, comparison of means was assessed by one- way ANOVA, followed by Tukey’s HSD test for post hoc comparisons. For biochemical analysis, a two-sample Kolmogorov-Smirnov test was performed for comparing two groups. For biomechanical analysis, comparison of means was assessed by one-way ANOVA and Tukey’s HSD test. When the data did not meet the requirements for a parametric test, a Kruskal-Wallis test was performed, followed by the Mann–Whitney test for post hoc comparisons. Values of p <0.05 were considered statistically significant. The mean and standard deviation (SD) are presented. 158 CHAPTER 8