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Figure 4. Histological staining of ear cartilage. (A) Sirius red, Resorcin-Fuchsin and Safranin-O staining of histological sections of native human ear cartilage for all ear regions and for young and old adult age groups. The high cell density of ear cartilage can be observed (B) . The lighter sirius red and Safranin-O staining in the old adult samples reflect the lower collagen and sGAG content measured by DMMB assay (see Figure 3). The Resorcin-Fuchsin staining demonstrates the high elastin content of the cartilage and the surrounding perichondrium. 37 EAR CARTILAGE CHARACTERISTICS 2