15502-m-pleumeekers

Contents List of abbreviations 6 Introduction 9 Chapter one General introduction 11 Thesis aim and outline 21 Cartilage properties 25 Chapter two Mechanical and biochemical mapping of human auricular cartilage for reliable assessment of tissue-engineered constructs 27 Chapter three Structural and mechanical comparison of human ear, alar and septal cartilage 43 Cell sources 57 Chapter four The in vitro and in vivo capacity of culture-expanded human cells from several sources encapsulated in alginate to form cartilage 59 Chapter five The trophic effect of adipose-tissue-derived and bone-marrow- derived mesenchymal stem cells on chondrocytes in co-culture 83 Chapter six Cartilage regeneration in the head and neck area: Combination of ear or nasal chondrocytes and mesenchymal stem cells improves cartilage production 107 Scaffolds 127 Chapter seven Preparation and characterization of a decellularized cartilage scaffold for ear cartilage reconstruction 129 Chapter eight Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo 147 Discussion and summary 173 Chapter nine Discussion and future perspectives for clinical application. 175 Chapter ten Summary 191 Chapter eleven Nederlandse samenvatting 197 References 203 Appendices 221 PhD portfolio 222 Publications 225 Dankwoord 227 Curriculum vitae 231